A rapid DNA cloning system is a research interest of many scientists. TA cloning is one of the methods used for the cloning of PCR-amplified DNA molecules. The TA cloning method is a convenient and labor-saving replacement to traditional, restriction enzyme-mediated cloning strategies. A T-vector called pBlueskript ΙΙ SK-1 with the lethal gene ccdB was designed to construct a positive selection...

objective: toxoplasmosis, caused by an intracellular protozoan parasite, and the toxoplasma gondii, is widespread throughout the world. in recent years, significant progress has been made in the identification of vaccine candidates which could induce a protective response. gra7, an excretory 29 kda toxoplasma gondii a dense granular antigen released by infected host cells. in tachyzoite-infect...

background: the homologous recombination (hr) is one of the conventional cloning methods for the production of recombinant dna. it is a quick method for in vivo dna cloning without using the high cost restriction enzymes. a few modifications in the cloning procedure can increase the efficiency of this method. m aterials and methods: in this study, effect of heating on the rate of the igg1 heavy...

background: angiogenesis is an important process in various physiologic and pathologic states. the most significant stimulator of angiogenesis is vascular endothelial growth factor (vegf). in contrast, vasohibin1 acts as an angiogenesis inhibitor which specifically inhibits new vessels formation. the aim of the present study was cloning and expression of vasohibin1 gene in e. coli as well as pu...

conclusions this is the first report of expression of cmv cp gene in iran, which is important for the preparation of anti-cmv antibody and paving the way for the use of the virus coat protein as a nanomaterial. results the identity of the expressed protein was confirmed by immunoassays such as western blot, dot-immunobinding assay (diba) and enzyme-linked immunosorbent assay (elisa) by the use ...

background & objective: streptococcosis is one of the bacterial infections in fish, especially rainbow trout which infects brain and nervous systems of fish and is caused by s. iniae . estimation of the impact of disease prevalence by s. iniae in fish farming in some countries is reported about 100 million dollars per year. some of the most effective proteins in pathogenicity of these bacteria ...

introduction: beta thalassemias are a heterogenous group of autosomal recessive disorders, characterized by reduced or absent production of the b-globin chain by the affected allele. transplantation of allogenic hematopoietic stem cells (hsc) is a curative approach but this therapeutic option is not available to the majority of patients. transplantation of genetically corrected autologous hsc i...

background: tuberculosis (tb) remains as a major cause of death around the world. construction of a new vaccine against tuberculosis is an effective way to control it. several vaccines against this disease have been developed. the aim of the present study was to cloning of tb10.4 gene in pcdna3.1+ plasmid and evaluation of its expression in eukaryotic cells. methods: firstly, tb10.4 fragment wa...

pistacia khinjuk (stocks) is a native species that, along with p. atlantica, is widely distributed from eastern to western iran through the makran zone, zagros mountains and the sanandaj-sirjan zone, ranging from 50 to 3300 m above sea level. the identification of resistance gene analogs holds great promise for developing resistant plants. a pcr approach with degenerate primers designed from co...